TEMPORAL AND SPATIAL ALTERATIONS IN UTERINE ESTROGEN-RECEPTOR AND PROGESTERONE-RECEPTOR GENE-EXPRESSION DURING THE ESTROUS-CYCLE AND EARLY-PREGNANCY IN THE EWE

Mechanisms regulating responses of the ovine uterus to endocrine and p aracrine signals during the estrous cycle and pregnancy are likely to require tissue- and cell-specific regulation of steroid hormone recept or gene expression. To determine effects of day and pregnancy status ( cyclic or pregnant) on uteri ne estrogen receptor (ER) and progesteron e receptor (PR) gene expression, ewes were hysterectomined either on D ay 1 (Day 0 = estrus/mating), 6, 11, 13, or 15 of the estrous cycle (n = 3/day) or on Day 11, 13, 15, 17, or 25 of early pregnancy (n = 5/da y), Steady state levels of ER and PR mRNA were determined in endometri al and myometrial tissues by slot-blot hybridization and ribonuclease protection assays, respectively, using homologous ovine ER and PR cRNA probes, Changes in spatial expression of ER and PR mRNA and protein i n uterine tissue sections were determined by in situ hybridization and immunocytochemical analyses. In cyclic ewes, steady state levels of e ndometrial ER mRNA were highest on Day 1, declined between Days 1 and 6, and increased between Days 11 and 15, However in pregnant ewes, end ometrial ER mRNA levels decreased between Days 11 and 15 and increased slightly between Days 15 and 25. In cyclic ewes, levels of myometrial ER mRNA were highest on Day 1, decreased to Day 6, and remained low t hereafter. In cyclic ewes, endometrial PR mRNA levels were highest on Day 1, decreased between Days 1 and 11, and then increased between Day s 13 and 15. In cyclic ewes, myometrial PR mRNA levels were highest on Day 1 and declined thereafter. Endometrial PR mRNA levels were not di fferent between cyclic and pregnant ewes on Days 11, 13, and 15. In pr egnant ewes, PR mRNA levels were low on Day 11, increased between Days 11 and 17, and decreased between Days 17 and 25. In pregnant ewes, my ometrial PR mRNA levels were low and did not change between Days 11 an d 25. In situ hybridization and immunocytochemical analyses revealed d istinct tissue- and cell type-specific alterations in uterine ER and P R mRNA and protein expression during the estrous cycle and early pregn ancy that generally paralleled overall changes in steady state levels of ER and PR mRNAs. In the endometrium, the most striking observation was that PR mRNA and protein expression disappeared from the luminal a nd shallow glandular epithelium between Days 6 and 13 of the estrous c ycle, whereas ER mRNA and protein expression was low on Days 6 and 11 and increased between Days 11 and 15 in the luminal and shallow glandu lar epithelium. During early pregnancy, expression of ER and PR mRNAs, as well as ER and PR protein, was very low or absent in the luminal a nd shallow glandular epithelium between Days 13 and 25 of pregnancy. M oreover, ER and PR mRNA and protein were consistently present at low l evels in the stroma and deep glandular epithelium in both cyclic (Days 11-15) and pregnant (Days 11-25) ewes. Collectively, results suggest that uterine ER and PR gene expression is regulated in a tissue- and c ell type-specific manner during the estrous cycle and early pregnancy.