IDENTIFICATION OF AMPLIFIED RESTRICTION FRAGMENT POLYMORPHISM (AFLP) MARKERS TIGHTLY LINKED TO THE TOMATO CF-9 GENE FOR RESISTANCE TO CLADOSPORIUM-FULVUM
Cm. Thomas et al., IDENTIFICATION OF AMPLIFIED RESTRICTION FRAGMENT POLYMORPHISM (AFLP) MARKERS TIGHTLY LINKED TO THE TOMATO CF-9 GENE FOR RESISTANCE TO CLADOSPORIUM-FULVUM, Plant journal, 8(5), 1995, pp. 785-794
Using the technique of amplified restriction fragment polymorphism (AF
LP) analysis, and bulked segregant pools from F-2 progeny of the cross
Lycopersicon esculentum (Cf9) x L. pennellii, approximately 42 000 AF
LP loci for tight linkage to the tomato Cf-9 gene for resistance to Cl
adosporium fulvum have been screened. Analysis of F-2 recombinants ide
ntified three markers which co-segregated with Cf-9. The Cf-9 gene has
recently been isolated by transposon tagging using the maize transpos
on Dissociation (Ds). Analysis of plasmid clones containing Cf-9 shows
that two of these markers are located on opposite sides of the gene s
eparated by 15.5 kbp of intervening DNA. AFLP analysis provides a rapi
d and efficient technique for detecting large numbers of DNA markers a
nd should expedite plant gene isolation by positional cloning and the
construction of high-density molecular linkage maps of plant genomes.