FROZEN STORED MURINE HYBRIDOMA CELLS CAN BE USED TO DETERMINE THE VIRULENCE OF LISTERIA-MONOCYTOGENES

Authors
BHUNIA AK WESTBROOK DG STORY R JOHNSON MG
Citation
Ak. Bhunia et al., FROZEN STORED MURINE HYBRIDOMA CELLS CAN BE USED TO DETERMINE THE VIRULENCE OF LISTERIA-MONOCYTOGENES, Journal of clinical microbiology, 33(12), 1995, pp. 3349-3351
Citations number
11
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
12
Year of publication
1995
Pages
3349 - 3351
Database
ISI
SICI code
0095-1137(1995)33:12<3349:FSMHCC>2.0.ZU;2-S
Abstract
Murine hybridoma cells, designated Ped-2E9, when stored up to 60 days at -196 degrees C or up to 48 days at -80 degrees C, gave results equi valent to those for freshly grown murine hybridoma cells in an in vitr o pathogenicity assay of Listeria species. Thus, laboratories do not n eed to have their own tissue culture facilities to maintain the hybrid oma cells for the assay described.