H. Zghal et al., DETERMINATION OF SUNSET YELLOW FCF AND PONCEAU 4R IN SWEETS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Sciences des aliments, 15(5), 1995, pp. 491-496
High-performance liquid chromatography with UV-visible detection was u
sed for the qualitative and quantitative determination of sunset yello
w FCF and ponceau 4R in sweets samples. The separation of dyes has bee
n obtained by reverse phase chromatography in a methanol/phosphate buf
fer system. Under these conditions, a good separation and short retent
ion times were obtained for each standard dye solution. Calibration cu
rves were linear. The applicability of this method has been shown with
two commercial sweets samples.