SIMPLE CONTROL OF REACTANTS DURING HYDROLYSIS OF TRIACYLGLYCEROLS

A HPLC method for rapid analysis by class of the components during oli ve oil enzyme hydrolysis is presented; Samples for analyses were taken directly from a reaction mixture without preparation. A single, short column with stationary phase RP18 and the RI detector were used. Elut ion was carried out under isocratic conditions with ternary mobile pha se composed of acetone, acetonitrile and isooctane. In the presence of remarkable amounts of water the optimum composition of the phase is 5 0:35:15 volume %. The quantitative results were obtained basing on num erical resolution of the partly overlapped peaks, assuming gaussian sh ape of the peaks. The analysis being rapid (12-15 min) and simple is s uitable for kinetic studies of the hydrolysis and/or synthesis of acyl glycerols with the immobilised enzyme catalyst.