The ability of two low resistance barrier filters (Collins DC-1 and Pa
ll Pf 305) to remove bacteria from expired air was assessed. A special
ly designed coupling device was used to hold each filter or a disposab
le plain cardboard mouthpiece a fixed distance (4.5 cm) from a blood a
gar plate. Volunteers performed maximal forced vital capacity manoeuvr
es through the assembled apparatus and bacteria impinged on to the aga
r plate were enumerated. Both filters allowed the transmission of appr
oximately one-third of expired colony forming units. The efficacy of t
hese filters for reducing the likelihood of cross-infection during spi
rometry is not supported by this study.