A RAPID METHOD FOR ISOLATION OF RETINAL-PIGMENT EPITHELIAL-CELLS FROMRAT EYEBALLS

Authors
SAKAGAMI K NAKA H HAYASHI A KAMEI M SASABE T TANO Y
Citation
K. Sakagami et al., A RAPID METHOD FOR ISOLATION OF RETINAL-PIGMENT EPITHELIAL-CELLS FROMRAT EYEBALLS, Ophthalmic research, 27(5), 1995, pp. 262-267
Citations number
15
Categorie Soggetti
Ophthalmology
Journal title
Ophthalmic research → ACNP
ISSN journal
00303747
Volume
27
Issue
5
Year of publication
1995
Pages
262 - 267
Database
ISI
SICI code
0030-3747(1995)27:5<262:ARMFIO>2.0.ZU;2-K
Abstract
A new, simple method for isolating retinal pigment epithelial (RPE) ce lls from rat eyeballs was developed: incubation of the eyeballs in 0.1 % proteinase K solution. This method yielded an average of 4 x 10(4) v iable RPE cells from each Sprague-Dawley rat eyeball in 1 h. These RPE cells were hexagonal and had cytokeratin in their cytoplasm and numer ous microvilli on their surface, similar to RPE cells evaluated in viv o. The results of this study thus show that our method for incubating eyeballs in proteinase K can provide good-quality RPE cells in suffici ent quantities for study in a short time.