Md. Peiperl et al., DETERMINATION OF COMBINED BENZIDINE IN FD-AND-C YELLOW-NO-6 (SUNSET-YELLOW FCF), Food and chemical toxicology, 33(10), 1995, pp. 829-839
Samples from 67 manufactured lots of FD&C Yellow No. 6 (Sunset Yellow
FCF; Colour Index No. 15985) were analysed for combined benzidine. The
se samples were selected from those submitted to the US Food and Drug
Administration for certification between October 1991 and December 199
2 by 13 dye distributors. Dithionite was used to reduce any combined b
enzidine present in the form of azo or disazo dyes to free benzidine.
This reduction was followed by extraction, diazotization and coupling
with 2-naphthol-3,6-disulfonic acid disodium salt (R salt). The total
benzidine was quantified as benzidine-R salt disazo dye by HPLC with d
etection at 540 nm and a quantification limit of 10 ng benzidine/g FD&
C Yellow No. 6. Of the 67 samples analysed, 34 were found to contain m
ore than 10 ng combined benzidine/g. Of these, 30 samples were from on
e manufacturing company, including three of its subsidiaries. The leve
l of combined benzidine found ranged from 11 to 104 ng/g, except for o
ne sample containing 941 ng/g.