EFFECT OF ANTITUBERCULOUS DRUGS, ISONIAZID, PYRAZINAMIDE AND RIFAMPICIN, ON CHEMILUMINESCENCE OF THE HUMAN POLYMORPHONUCLEAR LEUKOCYTES

Luminol-dependent chemiluminescence (CL) was used to assay the effect of three antituberculous drugs [isoniazid (INH), pyrazinamide (PZA), r ifampicin (RM)] on the production of reactive oxygen species (ROS) by polymorphonuclear leukocytes (PMN) isolated from blood of 5 healthy do nors, Drugs were added directly to the medium in concentrations: INH 5 mu g/ml, PZA 40 mu g/ml, RM 7 mu g/ml. These concentrations correspon d to peak serum concentrations after usual doses, RM was found to inhi bit FMLP (N-formyl-methionyl-leucyl-phenylalanine)-induced CL response of PMN, PZA did riot influence tire metabolic activity of neutrophils , and INH stimulated PMN CL.