ANALYSIS OF SPLICE DONOR AND ACCEPTOR SITE FUNCTION IN A TRANSPOSABLEGENE TRAP DERIVED FROM THE MAIZE ELEMENT ACTIVATOR

Citation
L. Nussaume et al., ANALYSIS OF SPLICE DONOR AND ACCEPTOR SITE FUNCTION IN A TRANSPOSABLEGENE TRAP DERIVED FROM THE MAIZE ELEMENT ACTIVATOR, MGG. Molecular & general genetics, 249(1), 1995, pp. 91-101
Citations number
61
Categorie Soggetti
Genetics & Heredity",Biology
ISSN journal
00268925
Volume
249
Issue
1
Year of publication
1995
Pages
91 - 101
Database
ISI
SICI code
0026-8925(1995)249:1<91:AOSDAA>2.0.ZU;2-L
Abstract
Gene trap vectors have been used in insertional mutagenesis in animal systems to clone genes with interesting patterns of expression. These vectors are designed to allow the expression of a reporter gene when t he vector inserts into a transcribed region. In this paper we examine alternative splicing events that result in the expression of a GUS rep orter gene carried on a Ds element which has been designed as a gene t rap vector for plants. We have developed a rapid and reliable method b ased on PCR to study such events. Many splice donor sites were observe d in the 3' Ac border. The relative frequency of utilisation of certai n splice donor and acceptor sites differed between tobacco and Arabido psis. A higher stringency of splicing was observed in Arabidopsis.