DEVELOPMENT AND EVALUATION OF A SELECTIVE AND INDICATIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE FROM TONSILS

In order to isolate ActinobacillIus pleuropneumoniae from mixed bacter ial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strai ns of A. pleuropneumoniae. Following optimization of the media 96 isol ates of A. pleuropneumonine were tested on S-TSA and S-MBA. For isolat ion of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non -selective agars tested. Furthermore, contaminating growth by bacteria other than A. pleuropneumoniae was significantly reduced on S-MBA com pared to S-TSA and the two non-selective media.