D. Wang et Hs. Sul, UPSTREAM STIMULATORY FACTORS BIND TO INSULIN-RESPONSE SEQUENCE OF THEFATTY-ACID SYNTHASE PROMOTER - USF1 IS REGULATED, The Journal of biological chemistry, 270(48), 1995, pp. 28716-28722
Fatty acid synthase (FAS) plays a central role in de novo lipogenesis
in mammals, The concentration or activity of FAS in liver and adipose
tissue changes dramatically when animals are subjected to nutritional
and hormonal manipulations. We previously reported that due to changes
in transcription, FAS synthesis declines and increases in an insulin-
dependent manner during fasting and refeeding, respectively, and that
insulin administration of streptozotocin-diabetic mice stimulates FAS
transcription. We previously mapped the FAS insulin response sequence
(IRS) to the proximal promoter region from position -71 to position -5
0, which contains an E-box DNA binding motif, Here, using competition
gel shift assays and specific upstream stimulatory factor (USF) antibo
dies, we identified USF1 and USF2 as major components of complexes tha
t bind to the FAS IRS, W-cross-linking experiments further supported t
hat USFs bind the FAS IRS, We also found that the amount of the 43-kDa
USF1 was dramatically increased in liver of refed rats, In contrast,
the amount of USF2 remained the same in liver of fasted or refed rats,
Moreover, a 17-kDa protein in both fasted and refed rat liver was rec
ognized by anti-USF1 antibodies, and this 17-kDa USF1 related protein
was expressed in a manner opposite to that of the 43-kDa USF1, i.e. hi
gh in liver of fasted rats and decreased in liver of refed rats. These
data suggest that the regulation of USF expression mayplay an importa
nt role in the regulation of FAS transcription.