Jm. Kim et al., ANTIBACTERIAL ACTIVITY OF SOME ESSENTIAL OIL COMPONENTS AGAINST 5 FOODBORNE PATHOGENS, Journal of agricultural and food chemistry, 43(11), 1995, pp. 2839-2845
Antibacterial activity of 11 essential oil constituents against Escher
ichia coli, E. coli O157:H7, Salmonella typhimurium, Listeria monocyto
genes, and Vibrio vulnificus was tested at 5, 10, 15, and 20% in 1% Tw
een 20 using a paper disk method. Eight constituents were then tested
in liquid medium to determine minimum inhibitory and minimum bacterici
dal concentrations (MIC and MBC, respectively). V. vulnificus was most
susceptible using disk assay. Carvacrol showed strong bactericidal ac
tivity against all tester strains, while limonene, nerolidol, and beta
-ionone were mostly inactive. Carvacrol was highly bactericidal agains
t S. typhimurium and V. vulnificus in liquid medium (MBC 250 mu g/mL).
Citral and perillaldehyde had MBCs of 100 and 250 mu g/mL against V.
vulnificus. Terpineol and linalool were least potent against tester st
rains, with MBCs of 1000 mu g/ mL. Citral, geraniol, and perillaldehyd
e at 500 mu g/mL completely killed E. coli, E. coli O157:H7, and S. ty
phimurium, while citronellal at 250 mu g/mL killed V. vulnificus. Ther
efore, these compounds could serve as potential antibacterial agents t
o inhibit pathogen growth in food.