INTRACYTOPLASMIC SPERM INJECTION AND EMBRYO DEVELOPMENT OF HUMAN OOCYTES CRYOPRESERVED USING 1,2-PROPANEDIOL

Citation
Da. Gook et al., INTRACYTOPLASMIC SPERM INJECTION AND EMBRYO DEVELOPMENT OF HUMAN OOCYTES CRYOPRESERVED USING 1,2-PROPANEDIOL, Human reproduction, 10(10), 1995, pp. 2637-2641
Citations number
29
Categorie Soggetti
Reproductive Biology
Journal title
ISSN journal
02681161
Volume
10
Issue
10
Year of publication
1995
Pages
2637 - 2641
Database
ISI
SICI code
0268-1161(1995)10:10<2637:ISIAED>2.0.ZU;2-D
Abstract
This study reports the subsequent embryo development of cryopreserved mature human oocytes following insemination or intracytoplasmic sperm injection (ICSI). Metaphase II oocytes were cryopreserved using a slow freezing-rapid thawing procedure employing the cryoprotectant 1,2-pro panediol. The study was conducted at two centres. The normal inseminat ion of cryopreserved oocytes was undertaken in one centre, and ICSI of cryopreserved oocytes in the other. Both methods resulted in a 50% no rmal fertilization rate. A low rate of abnormal fertilization was obse rved in the inseminated group of oocytes (5%) compared with 21% for th e ICSI oocytes; this was not significantly different. Embryo developme nt was assessed daily for 7 days. All normal fertilized cryopreserved oocytes in both groups cleaved on day 2, with a similar appearance to in-vitro fertilization and ICSI embryos. In the normal inseminated ooc ytes, there was a significant decrease in the number of embryos cleavi ng on day 3 (33%) compared with the development of ICSI oocytes, with a subsequent gradual reduction over days 4 and 5 (22 and 11% respectiv ely) resulting in one early blastocyst on day 7 (11%). In contrast, al l ICSI-generated embryos continued to cleave on day 3, with a gradual reduction over subsequent days (day 4, 86%; day 5, 57%; day 6, 43%; da y 7, 29%). By day 7, two of the blastocysts had started to hatch, resu lting in a 66% hatching rate of blastocysts formed from ICSI of cryopr eserved oocytes. This is the first study to show normal development to the hatching blastocyst stage following ICSI of cryopreserved human o ocytes.