IN-VITRO METABOLISM OF 3,3',4,4'-TETRACHLOROBIPHENYL IN RELATION TO ETHOXYRESORUFIN-O-DEETHYLASE ACTIVITY IN LIVER-MICROSOMES OF SOME WILDLIFE SPECIES AND RAT
A. Murk et al., IN-VITRO METABOLISM OF 3,3',4,4'-TETRACHLOROBIPHENYL IN RELATION TO ETHOXYRESORUFIN-O-DEETHYLASE ACTIVITY IN LIVER-MICROSOMES OF SOME WILDLIFE SPECIES AND RAT, European journal of pharmacology. Environmental toxicology and pharmacology section, 270(2-3), 1994, pp. 253-261
A qualitative study was performed of the capacity of hepatic microsome
s of several wildlife species to metabolize 3,3',4,4'-tetrachlorobiphe
nyl (TCB). Hepatic microsomes of species environmentally exposed to po
lychlorinated biphenyls (PCBs): harbour porpoise (Phocoena phocoena),
harbour seal (Phoca vitulina), common tern (Stema hirundo), and hepati
c microsomes from species experimentally exposed to PCBs: elder duck (
Somateria mollissima), rainbow trout (Salmo gairdneri), flounder (Plat
ichthys flesus), and Wistar rat, were incubated with C-14-labelled TCB
([C-14]TCB). The mammals and birds were able to metabolize TCB at a r
ate that correlated with their ethoxyresorufin-O-deethylase (EROD) act
ivity. No [C-14]TCB metabolism was observed in the fish, despite eleva
ted EROD activity in the trout. HPLC analysis of diisopropylether extr
acts of the microsomal incubations indicated the presence of 4-OH-, 5-
OH-, and 6-OH-tetrachlorobiphenyl metabolites and a yet unidentified m
etabolite. The ratio of the different hydroxy metabolites formed varie
d for the various species.