A. Purwantara et al., EVALUATION OF METHODS FOR SCREENING OF CULTIVARS OF SUBTERRANEAN CLOVER (TRIFOLIUM-SUBTERRANEUM) FOR RESISTANCE TO PHYTOPHTHORA-CLANDESTINA, Australasian plant pathology, 24(3), 1995, pp. 179-187
Different methods of screening of cultivars of subterranean clover for
resistance to Phytophthora clandestina were evaluated. This involved
inoculation of peeled seeds and 3- and 10-day-old seedlings growing in
water agar with mycelial plugs and zoospore suspensions of the fungus
, and of seedlings growing in pasteurised potting mix with vermiculite
infested with the fungus. The resistance of four cultivars of subterr
anean clover to three races of the pathogen was determined by all meth
ods. The most reliable screening method was inoculation of seedlings g
rowing in pasteurised potting mix with infested vermiculite and record
ing relative severity of root rot 1 week after inoculation. Inoculatio
n, using either mycelial plugs or zoospore suspensions, of approximate
ly 10-day-old seedlings growing in water agar and measuring the length
of root discoloration and/or root colonisation can be regarded as an
alternative method which uses less space and materials.