ANALYSIS AND PRODUCTION OF 2 EXOPOLYSACCHARIDES FROM LACTOCOCCUS-LACTIS SUBSP CREMORIS LC330

Two polysaccharides produced concurrently by Lactococcus lactis subsp. cremoris strain LC330 have been identified. One had a high molecular mass (> 1 x 10(6) Da) and was neutral. The second was smaller (similar to 10000 Da), charged and had a high phosphorus content. Sugar compos ition also differed, In chemostat culture the neutral polysaccharide w as influenced by temperature and by nitrogen limitation. This polysacc haride was branched with terminal galactose moieties and contained gal actose, glucose and glucosamine. The phosphopolysaccharide was more co mplex with glucose, rhamnose, galactose and glucosamine in an approxim ate ratio of 6:5:4:1.