FAST PHOTOVOLTAGE MEASUREMENTS IN PHOTOSYNTHESIS .2. EXPERIMENTAL METHODS

Fast photovoltage measurements in the pico- and nanosecond time range represent a valuable tool for the study of excitation energy trapping, kinetics of charge separation, and the location of intermediary accep ters in the photosynthetic reaction center. How ever, data recording a nd data analysis are complex. In a preceding article (Wulf and Trissl, Biospectroscopy, 1 (1995), pp. xx-xx) we have described the theoretic al fundamentals of data analysis. Here we describe the experimental se t-up, calibration procedures, and strategies for the determination of the parameters involved. Limitations of the method will be discussed. (C) 1995 John Wiley & Sons, Inc.