Fast photovoltage measurements in the pico- and nanosecond time range
represent a valuable tool for the study of excitation energy trapping,
kinetics of charge separation, and the location of intermediary accep
ters in the photosynthetic reaction center. How ever, data recording a
nd data analysis are complex. In a preceding article (Wulf and Trissl,
Biospectroscopy, 1 (1995), pp. xx-xx) we have described the theoretic
al fundamentals of data analysis. Here we describe the experimental se
t-up, calibration procedures, and strategies for the determination of
the parameters involved. Limitations of the method will be discussed.
(C) 1995 John Wiley & Sons, Inc.