DELETION ANALYSIS OF GENE MINE WHICH ENCODES THE TOPOLOGICAL SPECIFICITY FACTOR OF CELL-DIVISION IN ESCHERICHIA-COLI

Citation
S. Pichoff et al., DELETION ANALYSIS OF GENE MINE WHICH ENCODES THE TOPOLOGICAL SPECIFICITY FACTOR OF CELL-DIVISION IN ESCHERICHIA-COLI, Molecular microbiology, 18(2), 1995, pp. 321-329
Citations number
26
Categorie Soggetti
Biology,Microbiology
Journal title
ISSN journal
0950382X
Volume
18
Issue
2
Year of publication
1995
Pages
321 - 329
Database
ISI
SICI code
0950-382X(1995)18:2<321:DAOGMW>2.0.ZU;2-2
Abstract
Division inhibition caused by the minCD gene products of Escherichia c oli is suppressed specifically at mid-cell by MinE protein expressed a t physiological levels. Excess MinE allows division to take place also at the poles, leading to a minicell-forming (Min(-)) phenotype, In or der to investigate the basis of this topological specificity, we have analysed the ability of truncated derivatives of MinE to suppress eith er minCD-dependent division inhibition in a chromosomal Delta(minB) ba ckground, or the division inhibition exerted by MinCD at the cell pole s in a minB(+) strain. Our results indicate that these two effects are not mediated by identical interactions of MinE protein, In addition, gel filtration and the yeast two-hybrid system indicated that MinE int eracts with itself by means of its central segment, Taken together, ou r results favour a model in which wild-type MinE dimer molecules direc t the division inhibitor molecules to the cell poles, thus preventing polar divisions and allowing non-polar sites to divide. This model exp lains how excess MinE, or an excess of certain MinE derivatives which prevent the accumulation of the division inhibitor at the poles, can c onfer a Min(-) phenotype in a minB(+) strain.