Hf. Rosenberg et Kd. Dyer, HUMAN RIBONUCLEASE-4 (RNASE-4) - CODING SEQUENCE, CHROMOSOMAL LOCALIZATION AND IDENTIFICATION OF 2 DISTINCT TRANSCRIPTS IN HUMAN SOMATIC TISSUES, Nucleic acids research, 23(21), 1995, pp. 4290-4295
We have isolated a unique genomic fragment encoding human ribonuclease
4 (RNase 4) of the mammalian ribonuclease gene family, whose members
include pancreatic ribonuclease, eosinophil-derived neurotoxin, eosino
phil cationic protein and angiogenin. We have determined that the codi
ng sequence of RNase 4 resides on a single exon found on human chromos
ome 14, The mRNA encoding RNase 4 was detected by Northern analysis in
a number of human somatic tissues, including pancreas, lung, skeletal
muscle, heart, kidney and placenta, but not brain; liver represents t
he most abundant source, Interestingly, the mRNA encoding RNase 4 is s
imilar to 2 kb in length, which is approximately twice as large as the
mRNAs encoding other members of this gene family, A larger (similar t
o 2,4 kb), second transcript was detected in hepatic, pancreatic and r
enal tissues, The similar to 2 kb RNase 4 mRNA was detected in cells o
f the human promyelocytic leukemia line, HL-60, that had been treated
with dibutyryl-cAMP to promote neutrophilic differentiation, In contra
st, no mRNA encoding RNase 4 could be detected in cells treated with p
horbol myristic acid (PMA), an agent promoting differentiation toward
monocyte/macrophages, suggesting the existence of elements regulating
tissue specific expression of this gene.