NMR studies of rimocidin, consisting of DQF-COSY, ROESY, HSQC, HMBC an
d 1D-TOCSY experiments, resulted in the assignment of the absolute con
figuration of the rimocidin chiral centers as 2S, 3R, 9S, 11R, 13S, 14
R, 15S, 17R and 27R. The geometry of tetraene chromophore was found to
be all-trans.