ELECTROPHORETIC ANALYSES ON THE PROTEOLYT IC DECOMPOSITION OF FSH PREPARATIONS

We investigated the influence of some proteolytic enzymes being presen t in the digestive canal (pepsin, trypsin, chymotrypsin) on the digest ion of porcine FSH-samples by electrophoresis. Trypsin and Chymotrypsi n digested the 18-kDa-protein much faster than pepsin, whereas a 67-kD a-protein was much faster digested by Pepsin than by Trypsin or Chymot rypsin. The overall proteolytic effect of Chymotrypsin is small compar ed with Pepsin and Trypsin. A subsequent digestion with Pepsin, Trypsi n and Chymotrypsin led to a complete proteolysis of all proteins being present in the sample.