Pc. Pandey et Hh. Weetall, EVANESCENT FLUOROBIOSENSOR FOR THE DETECTION OF POLYAROMATIC HYDROCARBON BASED ON DNA INTERCALATION, Applied biochemistry and biotechnology, 55(2), 1995, pp. 87-94
A flow-injection analysis (FIA) system coupled with an evanescent wave
(EW) biosensor employing total internal reflection of fluorescence ra
diation (TIRF) for the detection of polyaromatic hydrocarbon that inte
rcalates into DNA is reported. A highly fluorescent intercalator, ''et
hidium bromide,'' has been used as the reference compound for detectio
n. The EW biosensor was developed according to the procedure described
earlier (1,2). Data on the analysis of Naphthalene, 3-methylcholanthr
ene, 7,12-dimethylbenz(a)anthracene, 1,2-benzanthracene, and some stan
dard reference materials supplied by the National Institute of Standar
ds and Technology are reported. The relative ability of the polyaromat
ic hydrocarbon to displace ethidium bromide, based on the relative bin
ding ratio, is found to be on the order of 7,12-dimethylbenz[a]anthrac
ene > 3-methylcholanthrene > 1,2-benzanthracene > napthalene.