A. Hidas, HETEROCHROMATIN HETEROGENEITY REVEALED BY RESTRICTION-ENDONUCLEASE DIGESTION AND SUBSEQUENT C-BANDING ON BOVINE METAPHASE CHROMOSOMES, Hereditas, 122(3), 1995, pp. 285-288
Conventional alkaline C-banding was found to be an effective additiona
l step in restriction endonuclease banding to improve staining contras
t and to reveal hidden changes in the heterochromatic blocks. Examples
of these cases are demonstrated on the bovine chromosome set using Hi
nf I and Tag I endonucleases in the digestion experiments.