S. Sur et al., EOSINOPHILIC INFLAMMATION IS ASSOCIATED WITH ELEVATION OF INTERLEUKIN-5 IN THE AIRWAYS OF PATIENTS WITH SPONTANEOUS SYMPTOMATIC ASTHMA, Journal of allergy and clinical immunology, 96(5), 1995, pp. 661-668
In vitro and in vivo studies have shown an important role for interleu
kin-5 (IL-5) in regulating eosinophil proliferation, survival, and eff
ector function. Because eosinophilic inflammation is an important comp
onent of symptomatic episodes of asthma, we have investigated whether
increased levels of IL-5 protein are present in bronchoalveolar lavage
(BAL) fluid of patients with spontaneously symptomatic asthma (FEV(1)
, 61% predicted; FEF(25%-75%), 30% predicted) compared with patients w
ith asymptomatic asthma (FEV(1), 88% predicted; FEF(25%-75%), 76% pred
icted). The percent of BAL eosinophils (10.5% vs 0.6%) (p = 0.0001) an
d eosinophil-derived neurotoxin (386.0 ng/ml vs 6.3 ng/ml) (p = 0.0001
) was greater in BAL fluids derived from patients with symptomatic ast
hma compared with patients with asymptomatic asthma. Levels of IL-5 me
asured with an immunoradiometric assay were significantly higher in pa
tients with symptomatic asthma (n = 26) compared with those with asymp
tomatic asthma (n = 18) (274 pg/ml vs < 13 pg/ml) (p = 0.02). The incr
eased IL-5 levels were noted in a subset of patients with symptomatic
asthma with BAL absolute eosinophil counts greater than 10(6) (IL-5, 6
64 pg/ml; n = 10) as opposed to patients with symptomatic asthma with
BAL eosinophil counts less than 10(6) (IL-5, < 13 pg/ml; n = 16) (p =
0.005). This study Suggests that IL-5 is not only induced in experimen
tal models of allergen-induced asthma but can also be detected as asth
ma progresses from the asymptomatic to the clinically symptomatic stat
e in subjects with significant BAL eosinophilia.