SEQUENCES OF THE CDNAS ENCODING THE HEAVY-CHAIN AND LIGHT-CHAIN FAB REGION OF AN ANTIBODY TO THE PHENYLUREA HERBICIDE DIURON

Authors
BELL CW SCHOLTHOF KBG ZHANG GS KARU AE
Citation
Cw. Bell et al., SEQUENCES OF THE CDNAS ENCODING THE HEAVY-CHAIN AND LIGHT-CHAIN FAB REGION OF AN ANTIBODY TO THE PHENYLUREA HERBICIDE DIURON, Gene, 165(2), 1995, pp. 323-324
Citations number
4
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
165
Issue
2
Year of publication
1995
Pages
323 - 324
Database
ISI
SICI code
0378-1119(1995)165:2<323:SOTCET>2.0.ZU;2-P
Abstract
The cDNAs from a hybridoma (mAb 481.1) specific for diuron, a widely u sed phenylurea herbicide, were cloned into the phage display vector pC omb8. Antigen-binding clones were selected by panning on diuron-hapten -BSA conjugates. The nucleotide and deduced amino-acid sequences encod ing the Fab regions of the light (kappa) and heavy (gamma) chains were determined. The light chain was from mouse kappa chain subgroup III a nd the heavy chain was a member of the mouse H chain subgroup III(d).