Cw. Bell et al., SEQUENCES OF THE CDNAS ENCODING THE HEAVY-CHAIN AND LIGHT-CHAIN FAB REGION OF AN ANTIBODY TO THE PHENYLUREA HERBICIDE DIURON, Gene, 165(2), 1995, pp. 323-324
The cDNAs from a hybridoma (mAb 481.1) specific for diuron, a widely u
sed phenylurea herbicide, were cloned into the phage display vector pC
omb8. Antigen-binding clones were selected by panning on diuron-hapten
-BSA conjugates. The nucleotide and deduced amino-acid sequences encod
ing the Fab regions of the light (kappa) and heavy (gamma) chains were
determined. The light chain was from mouse kappa chain subgroup III a
nd the heavy chain was a member of the mouse H chain subgroup III(d).