ROLE OF T-CELLS, VIRUS NEUTRALIZING ANTIBODIES AND COMPLEMENT-MEDIATED ANTIBODY LYSIS IN THE IMMUNE-RESPONSE AGAINST EQUINE HERPESVIRUS TYPE-1 (EHV-1) INFECTION OF C3H (H-2(K)) AND BALB C (H-2(D)) MICE/
Dg. Alber et al., ROLE OF T-CELLS, VIRUS NEUTRALIZING ANTIBODIES AND COMPLEMENT-MEDIATED ANTIBODY LYSIS IN THE IMMUNE-RESPONSE AGAINST EQUINE HERPESVIRUS TYPE-1 (EHV-1) INFECTION OF C3H (H-2(K)) AND BALB C (H-2(D)) MICE/, Research in Veterinary Science, 59(3), 1995, pp. 205-213
The suitability of C3H (H-2(k)) and BALB/c (H-2(d)) mice for use as sm
all animal models in which to study immunity to EHV-I was assessed. An
in vitro T cell response mediated by both CD4+ and CD8+ T cells was d
etected both during the acute phase of infection and after challenge w
ith a second dose of EHV-1 at two months in lymphocyte populations tak
en from the spleens of both types of mouse. The responses were apparen
t until at least 61 days after the primary inoculation. After challeng
e, T cells from mice previously infected with EHV-1 responded by as ea
rly as day 3 after infection and higher levels of T cell proliferation
were reached than in mice undergoing a primary infection. Immunologic
al cross-reactivity with the closely related virus, EHV-4 was detected
and some activity against herpes simplex virus type-1 (HSV-1) was obs
erved during the acute phase of infection. T cell responses were detec
ted in the draining cervical lymph nodes but not in the inguinal lymph
nodes of the mice and these were the primary sites of T cell activati
on. Complement-dependent virus neutralising antibodies were present by
day 8 after infection. These antibodies were also able to lyse EHV-1
infected target cells in vitro. Complement-independent virus neutralis
ing antibodies were found before challenge only in C3H mice. The clini
cal signs and duration of virus shedding were reduced after challenge.
The time course of the appearance of the different immune effector me
chanisms is discussed in relation to the clearance of virus from the i
nfected mice. The results suggest that C3H mice provide a better model
in which to study potential vaccine candidates against EHV-1 infectio
ns of the horse than BALB/c mice.