EVALUATION OF A PURIFICATION PROCEDURE FOR THE MUSCARINIC RECEPTOR FOR THE PURPOSE OF QUANTITATIVE RECEPTOR ASSAYS OF ANTICHOLINERGICS .A. THE MEMBRANE-BOUND RECEPTOR

The presented purification procedure for the muscarinic receptor from calf striatum includes the extraction of lipids with hexane in the fir st step and the removal of 39% of non-receptor proteins with 2 M NaCl in the second step. The simplicity of such an approach to the purifica tion of the receptor warrants its use in the routine practice for quan titative purposes. The high affinity binding of tertiary H-3-dexetimid e (H-3-DEX) and quaternary H-3-N-methylscopolamine (H-3-NMS) is preser ved after the removal of irrelevant lipids and proteins from the P2-pe llet.