EVALUATION OF A PURIFICATION PROCEDURE FOR THE MUSCARINIC RECEPTOR FOR THE PURPOSE OF QUANTITATIVE RECEPTOR ASSAYS OF ANTICHOLINERGICS .A. THE MEMBRANE-BOUND RECEPTOR
J. Smisterova et al., EVALUATION OF A PURIFICATION PROCEDURE FOR THE MUSCARINIC RECEPTOR FOR THE PURPOSE OF QUANTITATIVE RECEPTOR ASSAYS OF ANTICHOLINERGICS .A. THE MEMBRANE-BOUND RECEPTOR, Preparative biochemistry, 25(4), 1995, pp. 197-221
The presented purification procedure for the muscarinic receptor from
calf striatum includes the extraction of lipids with hexane in the fir
st step and the removal of 39% of non-receptor proteins with 2 M NaCl
in the second step. The simplicity of such an approach to the purifica
tion of the receptor warrants its use in the routine practice for quan
titative purposes. The high affinity binding of tertiary H-3-dexetimid
e (H-3-DEX) and quaternary H-3-N-methylscopolamine (H-3-NMS) is preser
ved after the removal of irrelevant lipids and proteins from the P2-pe
llet.