U. Pliquett et al., CHANGES IN THE PASSIVE ELECTRICAL-PROPERTIES OF HUMAN STRATUM-CORNEUMDUE TO ELECTROPORATION, Biochimica et biophysica acta. Biomembranes, 1239(2), 1995, pp. 111-121
The stratum corneum (SC) is the main barrier to molecular and ionic tr
ansport across mammalian skin and has been extensively studied by othe
rs at low voltages (U-skin(t) < 10 V) in order to partially characteri
ze the skin. Here we used one or more exponential pulses (tau(pulse) =
1 ms) and a temperature of 25 +/- 2 degrees C acid found that the low
voltage passive electrical properties (impedance) change rapidly and
significantly if these pulses result in U-skin,U-0 > 40 V. In contrast
, the dynamic resistance (describing passive electrical behavior in a
nonlinear range) changes dramatically by application of pulses between
40 V and 80 V and then it settles at levels between 50 Ohm and 100 Oh
m. we also found that recovery of the low voltage electrical parameter
s after pulsing depends mainly on the voltage, and, for multiple pulse
protocols, on the number of pulses. For single pulses of U-skin,U-0 a
pproximate to 90 V or less the electrical recovery was almost complete
, returning to within 0.90 of the pre-pulse value. In contrast, larger
pulses result progressively in decreased recovery. The recovery for p
ulses > 90 V revealed several characteristic times, suggesting the inv
olvement of different processes. For multiple pulses with U-skin,U-0 >
130 V almost no recovery of the transdermal resistance, R(skin), was
evident (returning to < 0.10 of pre-pulse values), i.e., essentially p
ermanent changes in the stratum comeum occurred. This is similar to th
at of single bilayer membrane electroporation, for which a transition
from reversible to irreversible behavior occurs as transmembrane volta
ge is increased. Thus, these results are consistent with the hypothesi
s that 'high-voltage' pulses cause electroporation within the SC, i.e.
, that elevated transmembrane voltages result in creation of new aqueo
us pathways ('pores') across SC lipid regions.