HPLC ANALYSIS OF 5-FU AND FDUMP IN TISSUE AND SERUM

Tissue and serum concentrations of 5-fluorouracil (5-FU) and 5-fluoro- 2'-deoxyuridine 5' monophosphate (FdUMP) can be determined using a sim ple and sensitive reverse phase HPLC technique. Samples are extracted with ethyl acetate, evaporated to dryness, resuspended in the mobile p hase (water/methanol [85:15]), and injected into a C18 mu Bondapack co lumn (250mm x 30mm) with a flow rate of 1 ml/min. Both fluorescence (2 66 lambda Excitation and 350 lambda Emission) and UV (250 nm) are used for detection. Standard solutions of 5-FU and FdUMP produce a linear relationship (r(2) = 0.985) with a detection sensitivity of 50 pg Reco very following this extraction procedure is 78%. This analytical metho d is simple and convenient to use for the simultaneous detection of 5- FU and FdUMP. (C) 1995 Academic Press, Inc.