M. Hall et al., IDENTIFICATION OF THE MAJOR LIPOPROTEINS IN CRAYFISH HEMOLYMPH AS PROTEINS INVOLVED IN IMMUNE RECOGNITION AND CLOTTING, Biochemical and biophysical research communications, 216(3), 1995, pp. 939-946
Lipid-containing hemolymph proteins from males of the crayfish Pacifas
tacus leniusculus were isolated by density gradient ultracentrifugatio
n. Two major lipoproteins, one high density lipoprotein (HDL) and one
very high density lipoprotein (VHDL), were characterized. The HDL and
the VHDL were found to be identical to two proteins previously studied
for their roles in immune recognition and hemolymph clotting, namely
the beta-1,3-glucan binding protein and the clotting protein. These re
sults imply that crayfish lipoproteins have dual functions, and that t
hey are involved in immunity, hemolymph clotting, and lipid transport
in these animals. Also, the oxygen-transporting protein hemocyanin was
found to have a small lipid content. (C) 1995 Academic Press, Inc.