MITOGENIC RESPONSE TO TGF-BETA IN 3T3-F442A CELLS

In 3T3-F442A cells, TGF-beta caused cellular proliferation in a time a nd dose-dependent manner. TGF-beta induced cyclin D1 and cdk2 proteins in 3T3-F442A cells. The mitogenic effect of TGF-beta was specific in nature. The antimitogenic agent, hGH, inhibited the mitogenic effect o f TGF-beta and was associated with inhibition of cyclin D1 expression. The protein kinase c inhibitor, staurosporine, inhibited the mitogeni c effect of TGF-beta. Taken together, these results suggest that TGF-b eta affects expression levels of cell cycle-regulated proteins and its mitogenic effect is mediated through protein kinase C in 3T3-F442A ce lls. (C) 1995 Academic Press, Inc.