GLUCOSE-6-PHOSPHATE-DEHYDROGENASE FROM SACCHAROMYCES-CEREVISIAE IS A GLYCOPROTEIN

Authors
REILLY KE ALLRED JB
Citation
Ke. Reilly et Jb. Allred, GLUCOSE-6-PHOSPHATE-DEHYDROGENASE FROM SACCHAROMYCES-CEREVISIAE IS A GLYCOPROTEIN, Biochemical and biophysical research communications, 216(3), 1995, pp. 993-998
Citations number
15
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
216
Issue
3
Year of publication
1995
Pages
993 - 998
Database
ISI
SICI code
0006-291X(1995)216:3<993:GFSIAG>2.0.ZU;2-4
Abstract
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) p urified from Saccharomyces cerevisiae was subjected to PAGE analysis u nder both nondenaturing and denaturing conditions. The enzyme, identif ied by both activity staining and anti-yeast G6PD antibody immunoblott ing, was shown to contain carbohydrate using the highly specific perio dated-digoxigenin antidigoxigenin method which is diagnostic for glyco proteins. (C) 1995 Academic Press, Inc.