J. Buggy et Ce. Bauer, CLONING AND CHARACTERIZATION OF SENC, A GENE INVOLVED IN BOTH AEROBICRESPIRATION AND PHOTOSYNTHESIS GENE-EXPRESSION IN RHODOBACTER-CAPSULATUS, Journal of bacteriology, 177(23), 1995, pp. 6958-6965
The purple nonsulfur photosynthetic eubacterium Rhodobacter capsulatus
is a versatile organism that can obtain cellular energy by several me
ans, including the capture of light energy for photosynthesis as well
as the use of light-independent respiration, in which molecular oxygen
serves as a terminal electron acceptor. In this study, we have identi
fied and characterized a novel gene, senC, mutations in which affect r
espiration as well as the induction of photosynthesis gene expression,
The protein coded by senC exhibits 33% sequence identity to the yeast
nucleus-encoded protein SCO1, which is thought to be a mitochondrion-
associated cytochrome c oxidase assembly factor, Like yeast SCO1, SenC
is required for optimal cytochrome c oxidase activity in aerobically
grown R. capsulatus cells, We further show that senC is required for m
aximal induction from the puf and puh operons, which encode the struct
ural polypeptides of the light-harvesting and reaction center complexe
s.