Ra. Karron et al., A LIVE HUMAN PARAINFLUENZA TYPE-3 VIRUS-VACCINE IS ATTENUATED AND IMMUNOGENIC IN HEALTHY INFANTS AND CHILDREN, The Journal of infectious diseases, 172(6), 1995, pp. 1445-1450
The safety, infectivity, immunogenicity, and phenotypic stability of t
he cold-passaged (cp) candidate vaccine cp-45, a cold-adapted (ca), te
mperature-sensitive (ts) mutant of the JS strain of human parainfluenz
a virus type 3 (HPIV-3), was evaluated in 114 children 6 months to 10
years old in a randomized, placebo-controlled, double-blind trial. The
cp-45 vaccine was well tolerated when given intranasally to parainflu
enza virus type 3 (PIV-3)-seropositive and -seronegative children. Wit
h 10(4) or 10(5) TCID50 of cp-45 vaccine, 86% of seronegative vaccinee
s were infected, 83% of whom shed virus at a mean peak titer of 10(2.2
) pfu/ml. Virus present in respiratory specimens retained the ts pheno
type, and each of 86 PIV-3 isolates tested retained both the ca and ts
phenotypes. One dose of 10(5) TCID50 of vaccine induced a serum hemag
glutination-inhibiting antibody response in 81% of vaccinees; the geom
etric mean titer was 1:32. These studies indicate that the cp-45 HPIV-
3 vaccine is satisfactorily attenuated, infectious, immunogenic, and p
henotypically stable and merits further evaluation in infants and youn
g children.