FREQUENT DEVELOPMENT OF MURINE T-CELL LYMPHOMAS WITH TCR-ALPHA BETA(+), CD4(-)/8(-) PHENOTYPE AFTER IMPLANTATION OF HUMAN INFLAMMATORY BREAST-CANCER CELLS IN BALB/C NUDE-MICE/

Tumors developed quite frequently in some of the visceral organs, incl uding spleen and liver, in BALB/c nude mice upon subcutaneously xenogr afting surgical specimens from five different inflammatory breast canc er patients. All of these tumors developed within two and a half month s to one year after the subcutaneous inoculation of surgical specimens . From these tumors, five independent transplantable tumors, including tMK-2, tHK-1, tYK-1, tYK-2 and tTY-1 have been established. Chromosom e analysis, morphologic studies by light and electron microscopy and p henotype analysis indicated that these tumors are of mouse origin. The tMK-2 tumor was highly metastatic to the spleen and liver when it was subcutaneously transplanted into the right scapular region. In additi on, the region where the tMK-2 tumor cells were subcutaneously inocula ted showed an apparently inflammatory process represented by erythema. After subcutaneous inoculation into the right scapular region, tHK-1, tYK-1, 2, and tTY-1 tumors also metastasized to some of the visceral organs, including spleen and liver. From these tumors, in vitro cell l ines were established. The cells grew in a stromal-cell dependent mann er under in vitro culture conditions. The cells were again tumorigenic at the inoculated region and metastasized to various organs, includin g liver and spleen, of BALB/c nude mice. Histological examination reve aled that the tumors showed features of malignant lymphoma. Phenotypic ally, these five tumors expressed early T lymphocyte markers as reveal ed by anti-mouse anti-TcR alpha/beta, anti-CDS, CD4 and CD8 monoclonal antibodies. To our knowledge, these cell lines are the first T-cell l ines showing the phenotype of extrathymically differentiated T-cells i n the liver.