A THERMOSTABLE HYDROGEN-PEROXIDE SENSOR-BASED ON WIRING OF SOYBEAN PEROXIDASE

Authors
VREEKE MS YONG KT HELLER A
Citation
Ms. Vreeke et al., A THERMOSTABLE HYDROGEN-PEROXIDE SENSOR-BASED ON WIRING OF SOYBEAN PEROXIDASE, Analytical chemistry, 67(23), 1995, pp. 4247-4249
Citations number
19
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
67
Issue
23
Year of publication
1995
Pages
4247 - 4249
Database
ISI
SICI code
0003-2700(1995)67:23<4247:ATHSOW>2.0.ZU;2-9
Abstract
A thermostable soybean peroxidase-based biosensor was formed by cross- linking and electrically ''wiring'' the enzyme through a redox-conduct ing hydrogel to a glassy carbon electrode. At 65 degrees C the sensiti vity of the sensor decayed only at a rate of only <2%/h. The sensor ma intained, at 25 degrees C, the 1 A cm(-2) M(-1) sensitivity and the 0. 1-200 mu M dynamic range of the earlier ''wired'' horseradish peroxida se-based sensors.