ONLINE RAMAN-SPECTROSCOPY OF RIBONUCLEOTIDES PRECONCENTRATED BY CAPILLARY ISOTACHOPHORESIS

Normal Raman spectroscopy is used as an on-line detector for capillary isotachophoresis (ITP) of adenosine 5'-triphosphate, adenosine 5'-dip hosphate, and adenosine 5'-monophosphate in phosphate buffers, Preconc entration is from a 1 x 10(-2) M phosphate buffer (pH 7.5) into a lead ing electrolyte of 0.1 M KCl or Na2SO4, with a terminating electrolyte of 0.1 M 4-morpholinepropane-sulfonic acid, The ribonucleotides are c oncentrated to above 10(-2) M at the detection window, allowing measur ement of Raman spectra with 1 s integration, from starting concentrati ons of 5 x 10(-6) M or higher.