DESCRIPTION OF AN AUTOMATED-METHOD FOR THE IN-VITRO MEASUREMENT OF TRYPSINOGEN SECRETION FROM PANCREATIC SEGMENTS

Authors
ROMAN JIS TAPIA JA CALVO JJ GARCIA LJ
Citation
Jis. Roman et al., DESCRIPTION OF AN AUTOMATED-METHOD FOR THE IN-VITRO MEASUREMENT OF TRYPSINOGEN SECRETION FROM PANCREATIC SEGMENTS, Analytical biochemistry, 232(1), 1995, pp. 129-132
Citations number
5
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
232
Issue
1
Year of publication
1995
Pages
129 - 132
Database
ISI
SICI code
0003-2697(1995)232:1<129:DOAAFT>2.0.ZU;2-9
Abstract
The characterization of trypsinogen output from superfused pancreatic tissue by an automated spectrophotometric method is described. To test the method we investigated the time-course and the dose-response curv e for acetylcholine-induced trypsinogen release from superfused pancre atic segments. We have demonstrated that this method allows the on-lin e detection and estimation of trypsinogen release with suitability, st ability, and sensitivity. (C) 1995 Academic Press, Inc.