RECOMBINANT-HUMAN-ERYTHROPOIETIN IMPROVES ENZYME-ACTIVITY IN ERYTHROCYTES OF PREDIALYSIS UREMICS

The influence of r-HuEPO on the activity of erythrocyte transketolase (ETKA), glutathione reductase (GSH) and glutamic pyruvic transaminase (EGPT) was determined by spectrophotometry. 150 IU/kg BW/week of r-HuE PO was given i.v, during 2 months to 12 predialyzed uremics (PDU). Twe nty healthy volunteers (HV) served as controls. GSH and EGPT activity were expressed as a coefficient. ETKA in HV = 2.39 +/- 0,10 and PDU = 1.53 +/- 0.10 pmol/ml/min differed significantly (p < 0.001). GSH in H V = 1.20 +/- 0.09 and PDU = 1.25 +/- 0.07, EGPT in HV = 1.20+0.10 and PDU = 1.38 +/- 0.11 differed significantly (p < 0.01 and p < 0.001, re spec tively). After 2 months of r-HuEPO treatment, ETKA, GSH and EGPT rose significantly (p < 0.01). Thus, r-HuEPO increases the activity of enzymes related with the content of vitamins B-1, B-2 and B-6 in urem ics.