NONSTARCHY POLYSACCHARIDE ANALYSIS OF COTYLEDON AND HULL OF LUPINUS-ALBUS

The cotyledon and hull of a lupin cultivar sample (Lupinus albus L.204 3N) were analyzed for polysaccharide composition. Nonstarchy polysacch arides (NSP) were obtained from the whole grain, hull, and cotyledon f ractions after defatting and pronase/alpha-amylase hydrolysis. Cotyled on polysaccharides of the water-insoluble fraction (11.7%) contained g alactose > arabinose > mannose. Cotyledon's water-soluble fraction (88 .3%) contained galactose > mannose > arabinose. The presence of galact ose and mannose suggested the presence of galactomannan. As expected, a high ratio of galactose to arabinose (3.3) in L. albus L2043N cotyle don indicated a high content of cell wall material. Fractions of cellu lose, hemicellulose A and B, anti oxalate-soluble material were separa ted by solubility properties. Arabinose was the dominant sugar in the hull and galactose in the cotyledon. The hull contained 56% NSP, most of which was hemicellulose, which represented 65% of the total NSP in the whole grain. The oxalate-soluble and cellulose fractions were not completely separated from hemicellulose, as indicated by the presence of arabinose and xylose in the former and arabinose in the latter.