Ki. Enomoto et al., RELEASE OF ARACHIDONIC-ACID VIA CA2-TUMOR CELLS( INCREASE STIMULATED BY PYROPHOSPHONUCLEOTIDES AND BRADYKININ IN MAMMARY), Cell biochemistry and function, 13(4), 1995, pp. 279-286
The relationship between the increase of intracellular Ca2+ and the re
lease of arachidonic acid by bradykinin and pyrophosphonucleotides was
studied in cultured mammary tumour cells, MMT060562. Bradykinin, ATP,
UTP and UDP induced an increase of intracellular Ca2+ and the release
of arachidonic acid from phospholipids into the extracellular fluid.
Release of arachidonic acid was also induced by the application of the
Ca2+ ionophore, A23187. Liberation of arachidonic acid by bradykinin
and ATP was reduced by mepacrine, a blocker of phospholipase A(2) and
W-7, a calmodulin antagonist. It is suggested that the increase in cyt
osolic Ca2+-induced release of arachidonic acid occurs through activat
ion of calmodulin-dependent phospholipase A(2).