Am. Desbene et al., UTILIZATION OF FLUORESCEIN SODIUM-SALT IN LASER-INDUCED INDIRECT FLUOROMETRIC DETECTION .2. APPLICATION TO ORGANIC-ANIONS, Journal of chromatography, 716(1-2), 1995, pp. 279-290
Citations number
51
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Previously, we have used the fluorescein sodium salt to generate the b
ackground signal in the indirect fluorimetric detection of inorganic c
ations (alkaline; alkaline earth metals and transition metals) separat
ed by capillary zone electrophoresis (CZE). In this work, we report th
at the use of fluorescein sodium salt can be extended to the detection
of organic anions. A study of the detection thresholds and of reprodu
cibility (elution times and effective electrophoretic mobilities) was
performed. As samples we used, on the one hand, a mixture of n-alkane
sulfonates (from C-1 to C-10) and, on the other hand, a mixture of car
boxylic acids (from C-1 to C-18). Under the optimal conditions, detect
ion thresholds of about 700 to 800 ppb were obtained and the reproduci
bility of effective electrophoretic mobilities was characterized by a
relative standard deviation of about 0.5%. Calibration curves were als
o established (correlation coefficients were found to be between 0.995
and 0.9998). Finally, we compared the evolution of the experimental r
esponse coefficients of these two series of samples as a function of a
lkyl chain length with theoretical response coefficients obtained from
findings reported by Ackermans et al. [J. Chromatogr., 549 (1991) 345
].