OPTICAL RESOLUTION OF AMINO-ACID DERIVATIVES BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH SODIUM N-TETRADECANOYL-L-GLUTAMATE

Optical resolution by micellar electrokinetic chromatography with sodi um N-tetradecanoyl-L-glutamate (STGlu) was investigated. Similar to th e case when using sodium N-dodecanoyl-L-valinate (SDVal), sodium N-dod ecanoyl-L-glutamate (SDGlu) and N-dodecanoyl-L-serine (DSer), addition of sodium dodecyl sulfate (SDS), urea and methanol-to STGlu micellar solutions gave improved peak shapes and enhanced the enantioselectivit y. With an ethylammonio]-1-propanesulfonate-SDS-urea-methanol solution , five phenylthiohydantoin-DL-amino acids were separated and each enan tiomeric pair was optically resolved. The resolution characteristic in the STGlu system, however, was not substantially different from those in SDVal, SDGlu and DSer systems.