CAPILLARY ELECTROPHORETIC METHODS FOR A CLEAR IDENTIFICATION OF SELENOAMINO ACIDS IN COMPLEX MATRICES SUCH AS HUMAN-MILK

Selenoamino acids from size-exclusionchromatographic fractions of huma n milk were identified and determined by capillary electrophoresis (CE ). For this purpose, different CE methods were developed to separate t hese selenoamino acids from other molecules with similar molecular mas ses. Methods were introduced for the clear identification of the analy tes. These methods were designed to allow the identification of seleno amino acids in spite of the presence of many molecules with similar mo bilities. Further, they overcome identification problems caused by shi fts of migration times (due to different ionic compositions) compared with standard solutions.