QUANTITATIVE H-1-NMR SPECTROSCOPY ANALYSIS OF THE POLY(3-HYDROXYALKANOATE) EXTRACTED FROM RHIZOBIUM-MELILOTI CELLS

Authors
JAN S ROBLOT C TAVERNIER P COURTOIS J COURTOIS B PORTAIS JC BARBOTIN JN SEGUIN JP
Citation
S. Jan et al., QUANTITATIVE H-1-NMR SPECTROSCOPY ANALYSIS OF THE POLY(3-HYDROXYALKANOATE) EXTRACTED FROM RHIZOBIUM-MELILOTI CELLS, Journal de chimie physique et de physico-chimie biologique, 92(10), 1995, pp. 1793-1796
Citations number
4
Categorie Soggetti
Biology,"Chemistry Physical
Journal title
Journal de chimie physique et de physico-chimie biologique → ACNP
Volume
92
Issue
10
Year of publication
1995
Pages
1793 - 1796
Database
ISI
SICI code
Abstract
H-1 NMR analysis was carried out to determine the nature and the conce ntration of the poly(3-hydroxyalkanoate) (PHA) accumulated by Rhizobiu m meliloti M5N1. The PHA was identified as being poly(3-hydroxybutyrat e) (PHB). Benzene was shown to meet all the requirements of an interna l reference for PHB quantification. NMR data were in good agreement wi th corresponding data obtained by Gas Chromatography (GC) analysis.