C. Srinivasan et al., DEMONSTRATION OF LACCASE IN THE WHITE-ROT BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM BKM-F1767, Applied and environmental microbiology, 61(12), 1995, pp. 4274-4277
It has been widely reported that the white rot basidiomycete Phaneroch
aete chrysosporium, unlike most other white rot fungi, does not produc
e laccase, an enzyme implicated in lignin biodegradation, Our results
showed that P. chrysosporium BKM-F1767 produces extracellular laccase
in a defined culture medium containing cellulose (10 g/liter) and eith
er 2.3 or 23 mM ammonium tartrate. Laccase activity was demonstrated i
n the concentrated extracellular culture fluids of this organism as de
termined by a laccase plate assay as well as a spectrophotometric assa
y with ABTS [2,2'-azinobis(3-ethylbenzathiazoline-6 acid)] as the subs
trate. Laccase activity was observed even after addition of excess cat
alase to the extracellular culture fluid to destroy the endogenously p
roduced hydrogen peroxide, indicating that the observed activity is no
t due to a peroxidase, Sodium dodecyl sulfate-polyacrylamide gel elect
rophoresis followed by activity staining with ABTS revealed the presen
ce of a laccase band with an estimated M(r) of 46,500.