IMMUNOHISTOLOGICAL DEMONSTRATION OF FELINE INFECTIOUS PERITONITIS VIRUS-ANTIGEN IN PARAFFIN-EMBEDDED TISSUES USING FELINE ASCITES OR MURINEMONOCLONAL-ANTIBODIES
R. Tammer et al., IMMUNOHISTOLOGICAL DEMONSTRATION OF FELINE INFECTIOUS PERITONITIS VIRUS-ANTIGEN IN PARAFFIN-EMBEDDED TISSUES USING FELINE ASCITES OR MURINEMONOCLONAL-ANTIBODIES, Veterinary immunology and immunopathology, 49(1-2), 1995, pp. 177-182
Feline infectious peritonitis (FIP) virus antigen was demonstrated aft
er methanol, ethanol or formalin fixation in paraffin-embedded tissues
by means of monoclonal and polyclonal antibodies. The monoclonal anti
body was induced by immunization with transmissible gastroenteritis vi
rus. Polyclonal antibodies were obtained by purification on protein A-
Sepharose of ascites fluid from a cat with FIP. Almost all cats diagno
sed as suffering from FIP by postmortem and histological examination e
xhibited FIP virus (FIPV) antigen in macrophages in granulomas whereas
FIPV antigen was only once demonstrable in another location.