IMMUNOHISTOLOGICAL DEMONSTRATION OF FELINE INFECTIOUS PERITONITIS VIRUS-ANTIGEN IN PARAFFIN-EMBEDDED TISSUES USING FELINE ASCITES OR MURINEMONOCLONAL-ANTIBODIES

Authors
TAMMER R EVENSEN O LUTZ H REINACHER M
Citation
R. Tammer et al., IMMUNOHISTOLOGICAL DEMONSTRATION OF FELINE INFECTIOUS PERITONITIS VIRUS-ANTIGEN IN PARAFFIN-EMBEDDED TISSUES USING FELINE ASCITES OR MURINEMONOCLONAL-ANTIBODIES, Veterinary immunology and immunopathology, 49(1-2), 1995, pp. 177-182
Citations number
15
Categorie Soggetti
Immunology,"Veterinary Sciences
Journal title
Veterinary immunology and immunopathology → ACNP
ISSN journal
01652427
Volume
49
Issue
1-2
Year of publication
1995
Pages
177 - 182
Database
ISI
SICI code
0165-2427(1995)49:1-2<177:IDOFIP>2.0.ZU;2-U
Abstract
Feline infectious peritonitis (FIP) virus antigen was demonstrated aft er methanol, ethanol or formalin fixation in paraffin-embedded tissues by means of monoclonal and polyclonal antibodies. The monoclonal anti body was induced by immunization with transmissible gastroenteritis vi rus. Polyclonal antibodies were obtained by purification on protein A- Sepharose of ascites fluid from a cat with FIP. Almost all cats diagno sed as suffering from FIP by postmortem and histological examination e xhibited FIP virus (FIPV) antigen in macrophages in granulomas whereas FIPV antigen was only once demonstrable in another location.