IMMUNOGOLD LOCALIZATION OF A DEVELOPMENTALLY-REGULATED, TAPETAL-SPECIFIC, 15 KDA LILY ANTHER PROTEIN

We have confirmed that the LLA-15 polypeptide of Lilium longiflorum is (a) tapetum specific with some expression possible in the adjacent mi ddle layer cells and (b) relatively abundant as evidenced by the high density of gold particles localized to the tapetal cells. We have esta blished that the protein is cytoplasmic and not associated with organe lles, membranes, extracellular matrix or wall. We also report an amino acid composition of the molecule and a partial sequence which bears n o resemblance to any protein yet described.