A SITE-SPECIFIC ENDONUCLEASE AND METHYLASE FROM THE THERMOPHILIC STRAIN OF BACILLUS SPECIES IS4

Authors
ZELINSKAYA NV KOVALEVSKAYA NP MATVIENKO NN ZHELEZNAYA LA MATVIENKO NI
Citation
Nv. Zelinskaya et al., A SITE-SPECIFIC ENDONUCLEASE AND METHYLASE FROM THE THERMOPHILIC STRAIN OF BACILLUS SPECIES IS4, Biochemistry, 60(9), 1995, pp. 1091-1102
Citations number
11
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
60
Issue
9
Year of publication
1995
Pages
1091 - 1102
Database
ISI
SICI code
0006-2979(1995)60:9<1091:ASEAMF>2.0.ZU;2-E
Abstract
A site-specific endonuclease (R . BspIS4I) and methylase (M . BspIS4I) have been isolated and purified to functional purity from the thermop hilic strain of Bacillus; species IS4. R . BspIS4I recognizes the sequ ence [GRAPHICS] and cleaves it as shown by arrows to form single-stran ded tetranucleotide 5'-protrud ing termini. The enzyme is an isoschizo mer of endonuclease BbvII. M . BspIS4I is attributed to the adenine-sp ecific methylases.